Bioconversion of animal hair waste using salt- and sulphide-tolerant Bacillus sp. KLP1 and depilation using keratinase

Keratinase is a potential industrial enzyme for handling keratinaceous solid wastes and also cleaner dehairing alternative to chemical methods in tanneries. The technologies towards circular economy approach are gaining importance leather manufacturing sector. In this study, new bacterial strain Bacillus sp. KLP1, exhibiting high tolerance sodium chloride sulphide, was isolated. This effectively used degrading the animal hair waste as biocatalyst removal of (dehairing) from sheep skins. completely degraded produced 2600 U/ml keratinase under optimized conditions within 72 h. crude exhibited an optimal activity at pH 9 60 °C. It showed 0.6 M NaCl 0.012 Na2S. keratinolytic protease complete addition, hydrolysed into free amino acids 16 These results clearly show use both skins hydrolysis addressing eco-processing management tanning industry. Thus, processing leads sustainable process through environmental-friendly holistic chemical-based processing.